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Original Article


Suitability of Papanicolaou stain for micronuclei mass screening in buccal smears comparing reference feulgen stain and papanicolaou stain in field

Suresh AminNilam PatelBharat Chattoo
Department of Genomic Centre, Maharaja Sayajirao University of Baroda, Gujarat, India
Corresponding Author:

Suresh Amin

Department of Genomic Centre, Maharaja Sayajirao University of Baroda, Gujarat, India
E-mail: suamin-_2001@yahoo.com

Corresponding Author:

Suresh Amin

Department of Genomic Centre, Maharaja Sayajirao University of Baroda, Gujarat, India
E-mail: suamin-_2001@yahoo.com

DOI:10.18203/issn.2456-3994.IntJMolImmunoOncol20184341

ABSTRACT


Background: Micronuclei (MN) assessment so far was done directly, individually, and intra- and, here, inter-observer bias could not be eliminated. It was subjective. Methods: In this study, accurate and reproducible MN counting was facilitated by the use of liquid cytology and image capturing with MN identification analysis. Results: Comparing the two nuclear staining techniques - DNA specific Feulgen stain and commonly available Papanicolaou (PAP) stain we found that both techniques gave statistically comparable accurate and reproducible MN results. T-test and non-parametric followed by paired non-parametric and then Wilcoxon matched-pairs signed rank test tell us that both the staining procedures have no difference at P <0.05. Conclusion: These protocols are comparable and reproducible, and may be adoptable for mass screening to assess the genotoxic effects of substances like tobacco.
Keywords: Field collection, Image capturing, Liquid buccal cytology, Micronuclei