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Original Article


Establishing etiopathogenesis in Epstein-Barr virus associated malignancies using chromogenic in situ hybridization

Ashwini PatkarJayamary J. LouisShaikhali M. BarodawalaKirti G. ChadhaAnuradha K. MurthyNikhil K. Majethia
Department of Surgical Pathology, Metropolis Healthcare, Mumbai, India
Corresponding Author:

Ashwini Patkar

Department of Surgical Pathology, Metropolis Healthcare, Mumbai, India
E-mail: shwini.patkar@gmail.com

Corresponding Author:

Ashwini Patkar

Department of Surgical Pathology, Metropolis Healthcare, Mumbai, India
E-mail: shwini.patkar@gmail.com

DOI:10.18203/issn.2456-3994.IntJMolImmunoOncol20172643

ABSTRACT


Background: Epstein–Barr virus is a human herpes virus ubiquitously infecting 90% of world population and causing 1% of tumors worldwide. The objectives were to establish etiopathogenesis in the Epstein-Barr virus (EBV) associated malignancies reported in our laboratory using the gold standard test, i.e., chromogenic in situ hybridization (CISH) for Epstein-Barr virus - encoded RNA (EBER). Aims and Objectives: We performed CISH on 244 cases of associated malignancies in the year 2013–2016, the decision was taken after H and E diagnosis and immunohistochemistry confirmation. Observation and Results: In the period of 2013–2016, 253 cases were evaluated by CISH for EBER. The age range in our study was 5–88 years with the mean age of 41.8 years, a slight male predominance was noted with male: female ratio of 1.5:1. CISH in 61 Classical Hodgkin lymphoma cases of 90cases were positive Conclusion: EBER ISH is the single best histochemical assay for defining EBV - related neoplasia. It is likely that emerging technologies such as gene expression profiling and proteomics will identify patterns of viral and human gene expression correlating with diagnosis, prognosis, and outcome in response to therapy.
Keywords: Chromogenic in situ hybridization, Epstein-Barr virus, Hodgkin’s lymphoma, Nasopharyngeal carcinoma